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Gannon University's alumni consist of a diverse group of individuals who have achieved notable accomplishments in their respective fields. Among them are:

• Mark L. Nelson, is a renowned chemist, scientist, and the inventor of Omadacycline, which is a tetracycline antibiotic.
• Robert J. Heibel, a retired FBI Agent who has dedicated his career to law enforcement.
• Joe Schember, who served as the 48th Mayor of Erie, Pennsylvania.
• Barbara Smith Warner is a distinguished member of the Oregon House of Representatives, representing District 45.
• James G. Zimmerly, a physician who played a pivotal role in the co-discovery of a meningococcal vaccine.
• Kevin Benson is a respected meteorologist known for his work at WPXI in Pittsburgh.